Constructing Heterogeneous MicrocomponentsOn an Electro-Microfluidic Platform
Synthesis and assembly of multi-functional, heterogeneous, and encoded microcomponents, or building blocks, are essential to engineering hierarchical, complex, and three--‐dimensional structures. The elaborately organized architectures devise metamaterials that hold new physical properties unusual in nature or artificial tissues that recapitulate physiological functions by imitating natural and biological arrangements. Here we demonstrate synthesis and assembly of microcomponents by electrowetting--‐on--‐dielectric (EWOD) and dielectrophoresis (DEP) on a robotic electro--‐microfluidic platform. The platform is capable of (1) driving different droplets of hydrogel prepolymers before cross--‐linking, (2) arranging particles or cells in the droplets, and (3) forming microcomponents by cross--‐linking droplets in different shapes. In the
presentation, we will demonstrate the synthesis and assembly of different hydrogel microcomponents containing different dyes and particles. The heterogeneous structures with cell adhesive GelMA (gelatin methacrylate) and
non--‐adhesive PEG--‐DA (Poly(ethylene glycol) diacrylate) surfaces are prepared for cell culture. The culture of fibroblasts NIH/3T3, neonatal mouse cardiomyocyte, and endothelia HUVEC on the engineered structures synthesized and assembled on the robotic electro-microfluidic platform will be reported.
Shih-Kang Fan received the B.S. degree from National Central University, Taiwan, in 1996, and the M.S. and Ph.D. degrees from the University of California, Los Angeles(UCLA), USA in 2001 and 2003, respectively.
Between 2004 and 2012, he was an Assistant Professor with the Institute of Nanotechnology at National Chiao Tung University (NCTU), Taiwan. From 2009 to 2012, he was an Associate Professor in the Institute of Nanotechnology, the Department of Material Science and Engineering, and the Department of Mechanical Engineering at NCTU. Science 2012, he has been an Associate Professor with the Department of Mechanical Engineering at National Taiwan University, Taiwan. His research interest focuses on electromicrofluidics.
Dr. Fan was a recipient of several young investigator awards in Taiwan, including the Yong Scholar's Creativity Award from Foundation for the Advancement of Outstanding Scholarship in 2014, the Research Award for Junior Research Investigators from Academia Sinica in 2012, and the Ta-You Wu Memorial Award from National Science Council in 2011.